Formulations and their Ingredients

Gamborg's B5 Medium : Gamborg, O. L., Miller, R. A., & Ojima, K. (1968). Nutrient requirements of suspension cultures of soybean root cells. Experimental cell research, 50(1), 151-158.


Name Concentration
Ammonium Sulfate 134.0 mg/L
Potassium Iodide 0.75 mg/L
Boric Acid 3.0 mg/L
Potassium Nitrate 2500.0 mg/L
Calcium Chloride, Anhydrous 113.24 mg/L
Sodium Dihydrogen Phosphate 150.0 mg/L
Cobalt Chloride Hexahydrate 0.025 mg/L
Zinc Sulfate Heptahydrate 2.0 mg/L
Copper(II) Sulfate Pentahydrate 0.025 mg/L
myo-Inositol 100.0 mg/L
Disodium EDTA Dihydrate 37.26 mg/L
Nicotinic Acid 1.0 mg/L
Iron(II) Sulfate Heptahydrate 27.8 mg/L
Pyridoxine Hydrochloride 1.0 mg/L
Magnesium Sulfate, Anhydrous 122.09 mg/L
Thiamine Hydrochloride 10.0 mg/L
Manganese(II) Sulfate Monohydrate 10.0 mg/L
Sodium Molybdate Dihydrate 0.25 mg/L